Features

InoMATRIX is a unique 3D scaffolding platform for use in cell culture, molecular biology and tissue engineering. InoMATRIX technology is based on electrospun nanofibers which enable topological and morphological mimicking of native ECM (extra cellular matrix). The 3D structure of InoMATRIX allows cell cultures to develop into tissues closely resembling native tissue.

Main features

  • The 3D like fibrous environment supports cell growth
  • High porosity and pore interconnection for optimal nutrient diffusion
  • Based on biocompatible and biodegradable polymers (e.g. PCL)
  • Regulated release of active substances for complex stimulation of cell processes (advanced nanofibers)
  • Simple manipulation and compatibility with microscopic and microplate based-assays
  • Ready-to-use kits in standard microplate formats (96 , 48 and 24 well plates)

InoMATRIX Morphology kit

ECM of different tissues show different organization of collagen fibers and provide a specific stimulation for tissue-specific primary and stem cells. Therefore, the nano-topology of artificial scaffolding material was shown to play important role for cell behavior during the cultivation period. The InoMATRIX Morphology kit delivers nanofiber systems prepared by electrospinning techniques, mimicking various ECM morphologies. The InoMATRIX Morphology kit offers up to date four different scaffolds made of poly-ɛ-caprolactone (PCL) polymer with different fibrous morphology:
  • nanofibrous scaffold with ̴ 200 nm fiber diameter
  • nano/microfibrous scaffold with two populations of fibers (thick microfibers and thin nanofibers)
  • microfibrous scaffold with ̴ 5 µm fiber diameter
  • nanoporous scaffold with nanostructured surface
TheInoMATRIX Morphology Selection platedelivers four scaffold types in one plate. The plate could be used for selection of scaffolds with optimal morphology, meeting the needs of specific experiments and particular cell type. After selecting the optimal scaffold morphology, the user may useInoMATRIX Morphology Single typeplate containing one type of scaffold with required morphology for further experiments. Principle of the InoMATRIX Morphology Selection plate kit

EXAMPLE: MG-63 human osteosarcoma testing on InoMATRIX Morphology Selection plate

MG-63cell line was tested on InoMATRIX Morphology Selection plate for 10 days. Cell proliferation and viability experiments showed high proliferation of cells on NF/MF scaffolds and viability of cells on all scaffolds during the whole cultivation period. Analysis of cellular morphology by SEM showed that cells on NF, NF/MF and POR showed well spreaded morphology. On the MF scaffolds the cells were assembled to spheroid-like structures mimicking avascular tumour environment.

InoMATRIX Morphology kit Selection Plates

InoMATRIX Morphology kit Selection plates are available in 24-, 48-, 96-well plates with attached InoMATRIX scaffolds. The microplates used may be delivered in cell-culture treated, non-treated polystyrene (non-adherent), black plates (fluorescence) and white plates (luminescence). On request the system may be delivered in form of bulk sheets (up to 40cm wide), custom sheets (e.g. for Petri-dishes) and re-usable well inserts (hanging well inserts). Thescaffolds could be coated with ECM proteins using simple protocols (e.g. laminin, collagen, fibronectin).
Cat. No Description
PCL-MORP01-96 Morphology kit Selection plate (24 x PCL NF, PCL MF, PCL NF/MF, PCL POR) in96 well plate
PCL-MORP01-96-W Morphology kit Selection plate (24 x PCL NF, PCL MF, PCL NF/MF, PCL POR) in white 96 well plate
PCL-MORP01-96-B Morphology kit Selection plate (24 x PCL NF, PCL MF, PCL NF/MF, PCL POR) in black 96 well plate
PCL-MORP01-48 Morphology kit Selection plate(12 x PCL NF, PCL MF, PCL NF/MF, PCLPOR) in 48well plate
PCL-MORP01-24 Morphology kit Selection plate(6 x PCL NF, PCL MF, PCL NF/MF, PCLPOR) in 24well plate

InoMATRIX Morphology kit Single type plates

InoMATRIX Morphology kit Single type plates are available in 24-, 48-, 96-well plates with attached InoMATRIX scaffolds. The microplates used may be delivered in cell-culture treated, non-treated polystyrene (non-adherent), black plates (fluorescence) and white plates (luminescence). On request the system may be delivered in form of bulk sheets (up to 40cm wide), custom sheets (e.g. for Petri-dishes) and re-usable well inserts (hanging well inserts). The scaffolds could be coated with ECM proteins using simple protocols (e.g. laminin, collagen, fibronectin).

Nanofibrous poly-ɛ-caprolactone (NF PCL) scaffolds

Electrospun scaffolds with nanofibrous morphology. The nanofibers are organized in random manner and form a fibrous mesh with small pores. The nanofibrous size of fibers supports cellular adhesion.
Morphology Fiber distribution
The scaffolds are formed by interconnected network of nanofibers. The analysis of fiber distribution shows, that majority of fibers has size up to 300 nm. The mean nanofiber diameter is 200 ± 70 nm. The fibers form pores with size about 1.5 µm.
Cat. No Description
PCL-NF01-96 Single type plate PCL NF (96 x PCL NF), 96-well
PCL-NF01-96-W Single type plate PCL NF (96 x PCL NF), white 96-well
PCL-NF01-96-B Single type plate PCL NF (96 x PCL NF), black 96-well
PCL-NF01-48 Single type plate PCL NF (48 x PCL NF), 48-well
PCL-NF01-24 Single type plate PCL NF (24 x PCL NF), 24-well

Nano/microfibrous poly-ɛ-caprolactone (NF/MF PCL) scaffolds

Electrospun scaffolds formed by microfibrous network filled with nanofibrous parts. The fibers are organized in random manner. The structure provides more 3D environment for cells by topologically rich surface.
Morphology Fiber distribution
The scaffolds are formed by interconnected network of micro and nanofibers. The scaffolds are composed of 2 types of fiber population. The nanofibers have broad size distribution with most dominant fraction about 300nm. The microfibrous part have mean diameter about 3-6 µm. The nano-microfibrous structures have bigger pore size (about 3µm) and higher roughness of scaffold surface.
Cat. No Description
PCL-NFMF01-96 Single type plate PCL NFMF (96 x PCL NFMF), 96-well
PCL-NFMF01-96-W Single type plate PCL NFMF (96 x PCL NFMF), white 96-well plate
PCL-NFMF01-96-B Single type plate PCL NFMF (96 x PCL NFMF), black 96-well plate
PCL-NFMF01-48 Single type plate PCL NFMF (48 x PCL NFMF), 48-well
PCL-NFMF01-24 Single type plate PCL NFMF (24 x PCL NFMF), 24-well

Microfibrous poly-ɛ-caprolactone (MF PCL) scaffolds

Electrospun scaffolds formed by microfibrous network. The fibers are organized in random manner. The structure provides 3D –like environment for cells by topologically rich surface and show bigger pore size.
Morphology Fiber distribution
The scaffolds are formed by interconnected network of microfibers with small fraction of nanofibers. The MF PCL scaffolds are composed of microfibers with mean size between 1-3 µm. The mesh contains also minor fraction of nanofibers with size about 750 nm. The microfibrous scaffold has pore size about 6 µm and 3D-like structure.
Cat. No Description
PCL-MF01-96 Single type plate PCL MF (96 x PCL MF), 96-well
PCL-MF01-96-W Single type plate PCL MF (96 x PCL MF), white 96-well plate
PCL-MF01-96-B Single type plate PCL MF (96 x PCL MF), black 96-well plate
PCL-MF01-48 Single type plate PCL MF (48 x PCL MF), 48-well plate
PCL-MF01-24 Single type plate PCL MF (24 x PCL MF), 24-well plate

Porous poly-ɛ-caprolactone (POR PCL) scaffolds

Compared to conventional fibers with smooth surface properties, the nanostuctured surface (nanopores) of POR PCL fibers result in higher roughness of fibers and increased absorption surface.
Morphology Fiber distribution
The scaffolds are formed by interconnected network of micro-and nanofibers with a nanoporous structure. The POR PCL scaffolds are composed of 2 types of iber population. The population of nanofibers have broad size distribution with most dominant fraction from 300 to 800 nm. The microfibrous part have mean diameter about 3-6 µm. The pores between fibers are about 3 µm.
Cat. No Description
PCL-POR01-96 Single type plate PCL POR (96 x PCL POR), 96-well plate
PCL-POR01-96-W Single type plate PCL POR (96 x PCL POR), white 96-well plate
PCL-POR01-96-B Single type plate PCL POR (96 x PCL POR), black 96-well plate
PCL-POR01-48 Single type plate PCL POR (48 x PCL POR), 48-well plate
PCL-POR01-24 Single type plate PCL POR (24 x PCL POR), 24-well plate