Large spectrum of bioactive molecules showing promise during in vitro testing is associated with activity loss during application. The reason may be connected with poor solubility, degradation by environmental conditions and bioelimination. For such reasons, the nano and microencapsulation provides a solution. The combination with suitable excipients, such as polymers and complex salts enables encapsulation, tight regulation of release and improves the stability of formulation. InoCure offers nanoencapsulation services based on its proprietary µSphere and InoMATRIX technologies. 

Advanced fibers for drug delivery

The unique feature of InoCure advanced nanofibrous products is a tunable release facilitating complex release profiles. The structure of fibers protects the embedded proteins, DNA, nanoparticles and drugs susceptible to environmental damage. Programmable release and mild immobilization enable production of next generation systems with complex release profiles.

Figure 1: Release regulation from InoMATRIX

InoMATRIX enables custom encapsulation of diverse macromolecules including: 
Application What can be delivered
Cell proliferation Growth factors (e.g. bFGF, PDGF, EGF)
Platelet rich plasma
Cell differentiation Growth factors
Differentiation media components
Tissue engineering Scaffolds for cell and cell-free tissue engineering
Molecular biology Organotypic models
Feeder replacement
siRNA release
Cosmetics Protection of active substances (e.g. hyaluronic acid, natural extracts, essential oils)
Stabilization of proteins and peptides
Bioactive non-woven textiles (e.g. masks, skin patches, wound dressings)
Food and nutraceuticals Vitamins and minerals
Fragrances
Protection against oxidation
The advanced fibers are prepared on request with custom range of active molecules. The fibers are available in form of bulk sheets (up to 40cm wide), 12-,24-, 48-, 96-well inserts or custom sheets and well inserts (CellCrown or hanging well inserts).

Nanoencapsulation by µSphere technology

The μSphere technology is based on electrohydrodynamic atomization, where the polymeric liquid is atomized by strong electric field. Such method enables production of small and uniform droplets resulting in uniform production of particles with size from 100nm to 10 μm. μSphere particles may serve as drug delivery vehicles.
The unique feature of our μSphere products is a tunable release facilitating complex release profiles. The core/shell structure of particles protects the embedded proteins, DNA and drugs susceptible to environmental damage. Programmable release and mild immobilization of active molecules enable production of next generation drug delivery systems applicable in pharmaceutical, nutrient and tissue engineering applications.
Nano and microparticles produced by µSphere technology enable encapsulation of diverse bioactive molecules. The technology enables encapsulation to both monolithical (single phase) and multi-material (multi-phase) systems. The technology is especially useful for applications where the produced material have to be easily dispersed into various oils, creams, and powders or applied to systemic administration. 

Figure 2: Core/shell µSphere nanoparticles
The key application areas of uSphere technology are:
Industry/field What can be delivered
Diet supplements Vitamins & minerals
Fitness and body building supplements
Probiotic cultures
Regulated delivery of plant extracts
Cosmetic use Small molecules
Hyaluronic acid
Pharmaceutical use Anticancer drugs
Cardio-logical drugs
Antibiotics
Ocular drug delivery
Tissue engineering Composite foams
Composite hydrogels
Nano/microparticles scaffolds
Technical use Combination with nanoparticles and inorganic materials
Encapsulation of fluorescent dyes and nanoparticles for flow cytometry and cell biology imaging
Formation of coatings (e.g. UV protection, nanostructured coatings)
The µSpheres are prepared on request with custom range of active molecules. The nano/microparticles are delivered in dry or dispersed form according to user`s requirements.

Upscaling and development

Development of protocols for electrospinning and electrohydrodynamic atomization of custom materials

InoCure Company offers optimization of electrospinning and electrohydrodynamic protocols for custom polymeric materials. We solve the problem with formulations to obtain the highest desired performance of your polymer.
The protocol development includes following phases:
  1. Detailed discussion of your requirements with our professional team (scientist working with nanofibers, medical device manufacturing, cell biology, tissue engineering).
  2. Finding possible solutions and electrospinning strategies. Verification of strategies and production of the primary batch.
  3. Characterization by wide set of methods (e.g. microscopy, analytical methods, mechanical testing) and report on electrospinning possibilities.
  4. Pre-production batch of the selected formulation.

All information and protocols are confidential between our customers and InoCure Company.

Up-scaling of electrospinning and electrohydrodynamic atomization process

The step from needle-based electrospinning protocols to needle-less electrospinning protocols is often connected with need for adaptation of protocols. Saving of time and economical resources are critical for efficient development and progress of your project. InoCure uses high-throughput needleless electrospinning and helps up-scaling your electrospinning protocols. The protocol development includes following phases:
  1. Detailed discussion of your requirements with our professional team (scientist working with fibers, medical device manufacturing, cell biology, tissue engineering).
  2. Selection of optimal high-throughput electrospinning device (multineedle, Nanospider or custom spinneret) based on requirements of your final manufacturing technique (not all systems adapted for different applications).
  3. Adaptation of your electrospinning protocols to the selected needleless platform.
  4. Production of pre-production batch of selected formulation.

All information and protocols are confidential between our customer and InoCure.

Development and integration of 3D cell culture substrates to research devices

Development of special fibrous coatings to obtain realistic cell cultivation conditions InoMATRIX scaffolds may be optimized for use in bioreactors and microfluidic cell culture systems. InoMATRIX substrates may be combined with different size/shape of devices.
We are highly interested in development and manufacturing of substrates for any specific system. The membranes may be prepared from wide set of materials allowing adjusting of mechanical and surface properties.

Cooperation on research projects 

We offer cooperation on diverse research tasks in the field of electrospinning. We are willing to apply for H2020 and other national/international/regional projects.
We offer expertise in the following areas:
  • Development of nanofibers
  • Advanced drug-delivery design
  • Development of electrospinning systems and their customization
  • Tissue engineering
  • Stem cell biology
InoCure will be happy to participate as full partner or as a contract organization.